Biogenesis of purple membrane: Regulation of bacterio-opsin synthesis
نویسندگان
چکیده
منابع مشابه
Biogenesis of purple membrane: regulation of bacterio-opsin synthesis.
In the cell membrane of Halobacteria a photoreceptor protein, bacteriorhodopsin, forms a twodimensional hexagonal crystalline network within the lipid matrix [l-4] . This specialized domain of the membrane has been termed purple membrane [5,6]. Besides its attractiveness for bioenergetic studies, the purple membrane appears highly suited for studying the biogenesis of a membrane: Purple membran...
متن کاملBiosynthesis of purple membrane: control of retinal synthesis by bacterio-opsin.
as follows: 500 ml 230 medium 100 reciprocating movements per min for 5 days at Low purple membrane production is observed under conditions of sufficient aeration: cells were grown in Erlenmeyer flasks containing only 90 ml medium under otherwise in the presence of mM nicotine yielded cells with ml medium) or (90 ml of bacteria-opsin, respectively (‘nicotine [7 ] Basal salt solution contained l...
متن کاملBacterio-opsin mutants of Halobacterium halobium.
The bacterio-opsin (bop) gene of Halobacterium halobium R1 has been cloned with about 40 kilobases of flanking genomic sequence. The 40-kilobase segment is derived from the (G+C)-rich fraction of the chromosome and is not homologous to the major (pHH1) or minor endogenous covalently closed circular DNA species of H. halobium. A 5.1-kilobase Pst I fragment containing the bop gene was subcloned i...
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We have examined transcripts corresponding to the Halobacterium halobium bacterio-opsin (BO) gene in wild-type and in BO-deficient mutant strains containing the insertion elements ISH1 or ISH2 in the BO gene. BO mRNA from the wild-type strain was purified by hybrid selection using single-stranded cDNA. Labeling by vaccinia virus capping enzyme and [alpha-(32)P]GTP showed that it contains the 5'...
متن کاملStudies on the biosynthesis of bacterio-opsin. Demonstration of the existence of protein species structurally related to bacterio-opsin.
1. The kinetics of processing newly synthesized bacterio-opsin from the non-crystalline state within the brown membrane to the crystalline state within the purple membrane was followed by pulse-chase experiments. 2. Biosynthesis of bacterio-opsin was found to be highly resistant to RNA-synthesis inhibitors like rifampicin and ethidium bromide. In the presence of ethidium bromide, only five prot...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1976
ISSN: 0014-5793
DOI: 10.1016/0014-5793(76)80673-4